The use of FCwSS in classifying the data represents the most significant difference from how the maps were processed originally Brown et al. Each dot represents a gene genes in total and genes are color-coded according to functional groups, as in Figure 1A. Consistent with this idea, we found that upregulation of Hsf1 target genes was either abolished or strongly reduced Figure 4E,F,G ; Supplementary file 4 when Top1 or Top1 and Top2 were degraded following nuclear depletion of Ifh1, indicating that RP production is required for Hsf1 target gene activation during RASTR.
The results are published today July 29 in Nature. For example, ribosomes could be altered to produce proteins from synthetic amino acids that resist degradation.
What is the function of the endoplasmic reticulum? Our data support a model in which the levels of newly synthetized RPs, imported into the nucleus but not yet assembled into ribosomes, work to continuously balance Hsf1 and Ifh1 activity, thus guarding against proteotoxic stress during ribosome assembly.
The effect on Isfh1, however, was unexpected. Heat shock factor 1 Hsf1 is a central actor in Protein Quality Control PQC and protein homeostasis proteostasis in eukaryotes, in both stressed and unstressed cell, and in pathological situations Li et al.